农林废弃物资源化利用栽培灵芝技术进展

尹哲玉; YIN Zheyu; 王家辰; WANG Jiachen; 亓香凝; QI Xiangning; 王建城; WANG Jiancheng; 白志辉; BAI Zhihui

doi:10.48014/pceep.20240315002

农林废弃物资源化利用栽培灵芝技术进展

The Progress of Ganoderma Lucidum Cultivation by Utilizing Agricultural and Forestry Wastes

中国生态环境保护进展 2024年第2卷第2期页码[11-21] 下载全文[0.7MB]

摘要

农林废弃物是我国重要且丰富的生物质资源之一, 通过绿色低碳的生物手段实现农林废弃物资源化利用具有重要意义, 有利于实现“碳中和”目标并推动农林产业的可持续发展。灵芝 (Ganoderma lucidum) 富含多种活性成分, 具有极高的药用价值, 因此, 灵芝的人工栽培和产品研发领域在全球范围内受到广泛关注。随着灵芝人工栽培产业规模的扩大, 利用农林废弃物作为灵芝栽培代料的方式是目前的研究热点。本文综述了在灵芝代料栽培领域各种农林废弃物转化利用的研究成果和应用案例, 并讨论了芝农/芝林栽培模式的发展潜力及优势。通过对农林废弃物在灵芝栽培中资源化利用的研究成果进行分析, 重点探讨了代料研发方案以及不同培养基质对灵芝生长效率、产量和营养成分的影响。以期为农林废弃物高值化利用以及人工灵芝栽培行业的发展提供参考, 并为农业和林业生态系统提升碳汇提供借鉴。

Abstract

Agricultural and forestry waste is one of the important and abundant biomass resources in China, and it is of great significance to realise the resource utilisation of agricultural and forestry waste through green and low-carbon biological means, which is conducive to achieving the goal of “carbon neutrality” and promoting the sustainable development of the agricultural and forestry industry. Ganoderma lucidum is rich in various active ingredients and has high medicinal value, so the artificial cultivation of Ganoderma lucidum and product research and development have received extensive attention worldwide. With the expansion of Ganoderma lucidum artificial cultivation industry, the use of agricultural and forestry wastes as a substitute material for Ganoderma lucidum cultivation is a hot research topic nowadays. This paper reviews the research results and application cases of various agricultural & forestry waste conversion using in the field of Ganoderma lucidum substitution cultivation and discusses the development potential and advantages of Ganoderma lucidum cultivation and combined cultivation mode of agriculture and forestry. By analyzing the research results on the resource utilization of agroforestry wastes in Ganoderma lucidum cultivation, we focused on the effects of culture media R&D protocols with different culture substrates, and its effect on the growth efficiency, yield and nutrient composition of Ganoderma lucidum. This provides reference for the high-value utilisation of agricultural and forestry wastes and the development of artificial Ganoderma lucidum cultivation industry, as well as the enhancement of carbon sinks in agricultural and forestry ecosystems.

DOI	10.48014/pceep.20240315002
文章类型	综述
收稿日期	2024-03-15
接收日期	2024-03-30
出版日期	2024-06-28
关键词	农林废弃物, 灵芝, 代料栽培, 资源化利用, 生态系统增汇
Keywords	Agricultural and forestry waste, Ganoderma lucidum, substitute cultivation, resource utilization, environmental protection
作者	尹哲玉^1,2, 王家辰^1,3,4, 亓香凝^1，2，3,, 王建城^3,, 白志辉^1,2
Author	YIN Zheyu^1,2, WANG Jiachen^1,3,4, QI Xiangning^1，2，3,, WANG Jiancheng^3,, BAI Zhihui^1,2
所在单位	1. 中国科学院生态环境研究中心, 北京 100085 2. 中国科学院大学资源与环境学院, 北京 100049 3. 滨州魏桥国科高等技术研究院, 滨州 256606 4. 郑州大学河南先进技术研究院, 郑州 450001
Company	1. Research Center for Eco-Environmental Science, Chinese Academy of Sciences, Beijing 100085, China 2. College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China 3. Binzhou Institute of Technology, Weiqiao-UCAS Science and Technology Park, Binzhou 256606, China 4. Zhengzhou University, Henan Institute of Advanced Technology, Zhengzhou 450001, China
浏览量	134
下载量	100
基金项目	本项研究得到了魏桥国科低碳技术专项(资助号GYY-DTFZ-2022-004); 国家重点研发计划课题(资助号 2023YFF1304101)、教育部“产学合作协同育人”项目(资助号BINTECH-KJZX-20220831-05)的资助。
参考文献	[1] 王学川, 王晓芹, 强涛涛. 生物质废弃物资源化利用研究进展[J]. 现代化工, 2015, 35(8): 63-66. https://doi.org/10.16606/j.cnki.issn0253-4320.2015.08.044. [2] 陈爽, 刘淑娟, 高悦, 等. 野生有柄灵芝生物学特性及驯化栽培[J]. 菌物学报, 2023, 42(11): 2218-2230. https://doi.org/10.13346/j.mycosystema.230059. [3] DU Z, DONG C H, WANG K, et al. Classification, Biological Characteristics and Cultivations of Ganoderma[M]// Ganoderma and Health: Biology, Chemistry and Industry. Singapore: Springer Singapore, 2019: 15-58. https://doi.org/10.1007/978-981-13-9867-4_2. [4] VEENA S S, PANDEY M. Paddy straw as a substrate for the cultivation of Lingzhi or Reishi medicinal Mushroom, Ganoderma lucidum(W. Curt. : Fr. )P. Karst. in India[J]. International Journal of Medicinal Mushrooms, 2011, 13(4): 397-400. https://dx.doi.org/10.1615/IntJMedMushr.v13.i4.100. [5] ZHOU X W. Cultivation of Ganoderma lucidum[M]// Edible and Medicinal Mushrooms. 2017: 385-413. https://doi.org/10.1002/9781119149446.ch18. [6] 金鑫, 刘宗敏, 黄羽佳, 等. 我国灵芝栽培现状及发展趋势[J]. 食药用菌, 2016, 24(1): 33-37. https://d.wanfangdata.com.cn/conference/9489313 [7] 康超, 王芳, 刘盈盈, 等. 我国农林废弃物栽培食用菌的研究进展[J]. 贵州农业科学, 2016, 44(1): 56-61. https://doi.org/10.3969/j.issn.1001-3601.2016.01.016. [8] 陆烝, 丁李春, 林忠宁等. 农林废弃物在灵芝栽培中的应用[J]. 南方农机, 2023, 54(24): 59-61. https://doi.org/10.3969/j.issn.1672-3872.2023.24.014. [9] BILGEN S, SARıKAYȦI. Utilization of forestry and agricultural wastes[J]. Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, 2016, 38(23): 3484-3490. https://doi.org/10.1080/15567036.2016.1169338. [10] 杨传文, 邢帆, 朱建春, 等. 中国秸秆资源的时空分布、利用现状与碳减排潜力[J]. 环境科学, 2023, 44(2): 1149-1162. https://doi.org/10.13227/j.hjkx.202201033. [11] 韩鲁佳, 闫巧娟, 刘向阳, 等. 中国农作物秸秆资源及其利用现状[J]. 农业工程学报, 2002(3): 87-91. https://doi.org/10.3321/j.issn:1002-6819.2002.03.022. [12] C'ILERDŽIC' J Lj, VUKOJEVIC' J B, KLAUS A S, et al. Wheat Straw - a promising substrate for Ganoderma lucidum cultivation[J]. Acta Scientiarum Polonorum Hortorum Cultus, 2018, 17(1): 13-22. https://doi.org/10.24326/asphc.2018.1.2. [13] KE L, WU Q, ZHANG D. Bioconversion of rape straw into a nutritionally enriched substrate by Ganoderma lucidum and yeast.[J]. African Journal of Biotechnology, 2011, 10(29): 5648-5653. https://api.semanticscholar.org/CorpusID:55634540 [14] 李林, 郭红艳, 孙晓杰, 等. 利用大豆秸秆和玉米秸秆栽培灵芝[J]. 食用菌学报, 2021, 28(4): 15-19. https://doi.org/10.16488/j.cnki.1005-9873.2021.04.003. [15] 伍琦, 谢晟, 朱模勇, 等. 利用棉花秸秆高产栽培灵芝技术的操作规程[J]. 棉花科学, 2017, 39(6): 27-28+31. https://doi.org/10.3969/j.issn.2095-3143.2017.06.008 [16] 辛世纪, 刘静雯, 班立桐, 等. 芦苇秸秆对灵芝液体发酵多糖产量的影响[J]. 中国食用菌, 2022, 41(4): 36-41. https://doi.org/10.13629/j.cnki.53-1054.2022.04.008. [17] GUPTA C. Nutraceutical potential of agri-horticultural wastes[J]. Obesity & Control Therapies: Open Access, 2017, 4(2): 1-8. http://dx.doi.org/10.15226/2374-8354/4/2/00137. [18] I. S E U, N. P K M, N. M. Evaluating the production of Ganoderma mushroom on corn cobs[J]. African Journal of Biotechnology, 2014, 13(22): 2215-2219. https://doi.org/10.5897/AJB2014.13650. [19] STABNIKOVA O, WANG J Y, IVANOV V. Valueadded biotechnological products from organic wastes[M]// Environmental Biotechnology. Totowa, NJ: Humana Press, 2010: 343-394. https://doi.org/10.1007/978-1-60327-140-0_8. [20] 隋文杰, 贾洪玉, 敬佩, 等. 中国果品加工固体废弃物资源化利用现状与分类管理研究[J]. 农业工程学报, 2018, 34(S1): 172-180. https://doi.org/10.11975/j.issn.1002-6819.2018.z.026. [21] 张长海, 沈汉国, 陈继敏, 等. 珠海灵芝栽培基质配方的筛选研究[J]. 中国农学通报, 2013, 29(13): 187-191. https://doi.org/10.11924/j.issn.1000-6850.2013-0731. [22] 韩嘉钰, 魏巍, 余梦瑶, 等. 不同栽培基质对灵芝基质营养物质及胞外酶活的影响[J]. 时珍国医国药, 2016, 27(3): 715-719. https://doi.org/10.3969/j.issn.1008-0805.2016.03.076. [23] 金鑫, 黄文丽, 李小林, 等. 不同果树枝条栽培灵芝基质配方研究[J]. 中国农学通报, 2015, 31(36): 156-160. https://doi.org/10.11924/j.issn.1000-6850.casb15070171. [24] HSIEH C, YANG F C. Reusing soy residue for the solid- state fermentation of Ganoderma lucidum .[J]. Bioresource technology, 2004, 91 1: 105-109. https://doi.org/10.1016/s0960-8524(03)00157-3 [25] 王允圃, 李积华, 刘玉环, 等. 甘蔗渣综合利用技术的最新进展[J]. 中国农学通报, 2010, 26(16): 370-375. https://doi.org/10.11924/j.issn.1000-6850.2010-1393 [26] BANGYEEKHUN E, NINLUAM N, POTIPRASERT W, et al. Cultivation of Lingzhi Mushroom, Ganoderma lucidum , by Using Sugarcane Bagasse[J]. Veridian EJournal, Science and Technology Silpakorn University, 2016, 3(6): 390-397. [27] 肖自添, 黄育江, 何焕清, 等. 适合孢子粉高产的赤灵芝栽培配方筛选[J]. 中国食用菌, 2023, 42(1): 74-79. https://doi.org/10.13629/j.cnki.53-1054.2023.01.013. [28] 赵风云, 杨鑫, 黄淑君, 等. 以蔗渣和棉籽壳为基质的灵芝酶活性及其营养对比研究[J]. 现代食品科技, 2015, 31(1): 84-89. https://doi.org/10.13982/j.mfst.1673-9078.2015.1.016. [29] YANG F C, HSIEH C, CHEN H M. Use of stillage grain from a rice-spirit distillery in the solid state fermentation of Ganoderma lucidum[J]. Process Biochemistry, 2003, 39(1): 21-26. https://doi.org/10.1016/S0032-9592(02)00255-8. [30] PEKSEN A, YAKUPOGLU G. Tea waste as a supplement for the cultivation of Ganoderma lucidum[J]. World Journal of Microbiology and Biotechnology, 2009, 25(4): 611-618. https://doi.org/10.1007/s11274-008-9931-z. [31] 杨健. 槐米渣在灵芝栽培料中的应用: CN201710009286. 3[P]. 2018-07-13. [32] 陈卫军, 任飞, 单丹, 等. 一种以芒果核为主料的灵芝栽培基质及制备方法: CN201910230123. 7[P]. 2019- 07-23. [33] 刘义军, 李盖, 林丽静, 等. 不同菠萝叶渣添加量对赤灵芝发酵基质营养成分及体外抗氧化活性研究[J]. 四川农业大学学报, 2024, 42(1): 197-206. https://doi.org/10.16036/j.issn.1000-2650.202306297. [34] GONZÁLEZ-MATUTE R, FIGLAS D, DEVALIS R, et al. Sunflower seed hulls as a main nutrient source for cultivating Ganoderma lucidum[J] Micologia Aplicada International, 2002, 14(2): 19-24. [35] OZCARIZ-FERMOSELLE M V, FRAILE-FABERO R, GIRBÉS-JUAN T, et al. Use of lignocellulosic wastes of pecan(Carya illinoinensis)in the cultivation of Ganoderma lucidum[J]. Revista Iberoamericana de Micología, 2018, 35(2): 103-109. https://doi.org/10.1016/j.riam.2017.09.005. [36] 肖自添, 刘明, 何焕清, 等. 咖啡渣栽培灵芝试验[J]. 食药用菌, 2015, 23(3): 196-198. [37] 赵秀芳. 醋渣中药渣栽培灵芝试验[J]. 食用菌, 1998(4): 21. [38] 方浩旭, 丁诗禧, 刘广明, 等. 竹笋壳在灵芝栽培生产中的应用[J]. 清远职业技术学院学报, 2017. https://doi.org/10.3969/j.issn.1674-4896.2017.01.011. [39] THIRIBHUVANAMALA G, KRISHNAMOORTHY A S. Evaluation of different lignocellulosic substrates for cultivation of medicinal mushroom Ganoderma lucidum[J]. Journal of Environmental Biology, 2021, 42(5): 1314-1319. https://doi.org/10.22438/jeb/42/5/MRN-1686. [40] C'ILERDŽIC' J, STAJIC' M, VUKOJEVIC' J, et al. Intraspecific diversity in the production and characterization of laccase within Ganoderma lucidum[J]. BioResources, 2014, 9(3): 5577-5587. https://doi.org/10.15376/biores.9.3.5577-5587. [41] C'ILERDŽIC' J, STAJIC' M, VUKOJEVIC' J. Activity of Mn-Oxidizing peroxidases of Ganoderma lucidum depending on cultivation conditions[J]. Bio Resources, 2015, 11(1): 95-104. https://doi.org/10.15376/biores.11.1.95-104. [42] SHI M, YANG Y, GUAN D, et al. Evaluation of solidstate fermentation by Ganoderma lucidum using soybean curd residue[J]. Food and Bioprocess Technology, 2013, 6(7): 1856-1867. https://doi.org/10.15376/biores.11.1.95-104. [43] ABID K, BOUDAGGA S, ABID O, et al. Bioconversion of grape pomace waste into suitable alternative feed for ruminants with Pleurotus cornucopiae and Ganoderma resinaceum via solid-state fermentation bioprocess[J]. Biomass Conversion and Biorefinery, 2023: 1-10. https://doi.org/10.1007/s13399-023-04771-z. [44] NISA F T, ASGHER M, ANWAR Z, et al. Influence of culture conditions on ligninolytic enzymes production from ganoderma lucidum using agro-industrial residues[J]. Fresenius Environmental Bulletin, 2014, 23(12): 3441-3447. [45] 刘瑜, 戚智勇, 赵佳颖, 等. 我国城市园林废弃物及其资源化利用现状[J]. 再生资源与循环经济, 2020, 13(8): 38-44. https://dx.doi.org/10.3969/j.issn.1674-0912.2020.08.009. [46] 徐杨, 杜祥哲, 齐英杰, 等. 浅析木材加工剩余物的利用途径[J]. 林产工业, 2015, 42(5): 40-44. https://doi.org/10.19531/j.issn1001-5299.2015.05.011. [47] 侯瑞宏, 廖森泰. 我国灵芝人工栽培研究进展[J]. 广东农业科学, 2009(11): 29-32. http://doi.org/10.3969/j.issn.1004-874X.2009.11.007. [48] Azizi M, Tavana M, Farsi M, et al. Yield performance of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum(W. Curt. : Fr. )P. Karst.(Higher Basidiomycetes), Using Different Waste Materials as Substrates [J]. International Journal of Medicinal Mushrooms, 2012, 14(5): 521-527. https://doi.org/10.1615/IntJMedMushr.v14.i5.110. [49] ROY S. Artificial cultivation of Ganoderma lucidum(Reishi Medicinal Mushroom)using different sawdusts as substrates[J]. American Journal of BioScience, 2015, 3(5): 178. https://doi.org/10.1615/intjmedmushr.v14.i5.110. [50] GURUNG O K, BUDATHOKI U, PARAJULI G. Effect of different substrates on the production of Ganoderma lucidum(Curt. : Fr. )Karst.[J]. Our Nature, 2013, 10(1): 191-198. https://doi.org/10.3126/ON.V10I1.7781. [51] 陈继敏, 潘丽晶, 张妙彬, 等. 芒果木屑栽培灵芝试验[J]. 中国瓜菜, 2013, 26(3): 43-45. https://doi.org/10.16861/j.cnki.zggc.2013.03.016. [52] PETRE M, TEODORESCU R I, STANICA F, et al. Mycotechnology for valorisation of fruit trww wastes as eco-friendly and useful products[J]. Agrolife Scientific Journal, 2022, 11(2): 130-135. https://doi.org/10.17930/AGL2022217 [53] 刘新华, 吕军, 董学堂, 等. 以果树枝木屑为主料灵芝和平菇轮作探索与实践[J]. 中国食用菌, 2017, 36(3): 88- 89+92. https://doi.org/10.13629/j.cnki.53-1054.2017.03.022. [54] 吴德峰, 涂建生, 叶财旺, 等. 竹粉灵芝仿野生栽培技术研究[J]. 玉林师范学院学报, 2016, 37(2): 70-74. https://doi.org/10.13792/j.cnki.cn45-1300/z.2016.02.014. [55] 李建科, 孟永宏, 刘柳, 等. 我国食品工业副产物资源化利用现状[J]. 食品科学技术学报, 2021, 39(6): 1-13. http://doi.org/10.12301/j.issn.2095-6002.2021.06.001 [56] 王红彦, 张轩铭, 王道龙, 等. 中国玉米芯资源量估算及其开发利用[J]. 中国农业资源与区划, 2016, 37(1): 1-8. [57] 陈远庆, 胡杰, 宋鹏, 等. 米糠粕的营养特性及其在饲料生产中的应用[J]. 饲料研究, 2022, 45(10): 155-159. https://doi.org/10.13557/j.cnki.issn1002-2813.2022.10.033. [58] 罗小锋, 江松颖. 棉籽壳价格变动轨迹及其对食用菌生产的影响———基于2010-2012年我国棉籽壳价格分析[J]. 华中农业大学学报(社会科学版), 2013(6): 59-64. https://doi.org/10.13300/j.cnki.hnwkxb.2013.06.014. [59] 张小琴, 黄昭密, 郑思芳, 等. 茶渣之再利用研究[J]. 福建茶叶, 2012, 34(6): 14-16. [60] 段新芳, 周泽峰, 徐金梅, 等. 我国林业剩余物资源、利用现状及建议[J]. 中国人造板, 2017, 24(11): 1-5. [61] BOH B, BEROVIC M, ZHANG J, et al. Ganoderma lucidumand its pharmaceutically active compounds[M]//EL-GEWELY M R. Biotechnology Annual Review: Elsevier, 2007: 265-301. https://doi.org/10.1016/s1387-2656(07)13010-6. [62] CHEN A W. Cultivation of the medicinal mushroomGanoderma lucidum(reishi)[J] The MushroomGrowers􀆳 Newsletter, 2002. https://www.mushroomcompany.com/resources/reishi/ReishionLogs.pdf [63] 韩卓琪. 不同代料栽培对灵芝的品质评价及多糖合成酶活性研究[D]. 长春: 吉林农业大学, 2022. [64] 韦会平, 刘正宇, 谭杨梅, 等. 农作物秸秆栽培灵芝的实验研究[J]. 中国农学通报, 2005, 21(11): 145-145. https://doi.org/10.11924/j.issn.1000-6850.0511145 [65] JANDAIK S, SINGH R, SHARMA M. Comparativegrowth characteristics and yield attributes of Lingzhior Reishi medicinal mushroom, Ganoderma lucidum(Higher basidiomycetes)on different substrates in India[J]. International Journal of Medicinal Mushrooms, 2013, 15(5): 497-503. https://doi.org/10.1615/intjmedmushr.v15.i5.70. [66] Mehta S, Jandaik S, Gupta D. Effect of cost-effectivesubstrates on growth cycle and yield of lingzhi or reishimedicinal mushroom, Ganoderma lucidum(higher Basidiomycetes)from Northwestern Himalaya(India)[J]. International Journal of Medicinal Mushrooms, 2014, 16(6): 585-591. https://doi.org/10.1615/intjmedmushrooms.v16.i6.80. [67] 刘明香, 林忠宁, 陈敏健, 等. 茶枝屑代料栽培对灵芝生物转化率和质量的影响[J]. 福建农业学报, 2011, 26(5): 742-746. https://doi.org/10.19303/j.issn.1008-0384.2011.05.012. [68] C'ILERDŽIC'J, VUKOJEVIC'J, STAJIC'M, et al. Biologicalactivity of Ganoderma lucidum basidiocarps cultivatedon alternative and commercial substrate[J]. Journal of Ethnopharmacology, 2014, 155(1): 312-319. https://doi.org/10.1016/j.jep.2014.05.036. [69] ATILA F, OGUTCU H, BILGINOGLU E, et al. Effect ofphenolic-rich forest and agri-food wastes on yield, antioxidant, and antimicrobial activities of Ganoderma lucidum[J]. Biomass Conversion and Biorefinery, 2023. https://doi.org/10.1007/s13399-023-04708-6. [70] 林忠宁, 陆烝, 林怡, 等. 茶园套种对灵芝生长和品质的影响[J]. 福建农业学报, 2020, 35(5): 532-537. https://doi.org/10.19303/j.issn.1008-0384.2020.05.010. [71] 高云超, 廖森泰, 肖更生, 等. “栽桑-养蚕-种菇”生态模式初探[J]. 广东农业科学, 2012, 39(22): 34-37. http://dx.doi.org/10.16768/j.issn.1004-874x.2012.22.052. [72] 李振皓, 李振宇, 李明焱, 等. 一种药稻轮作方法: CN202310871658. 9[P]. 2023-09-19. [73] 张玉梅, 蓝新隆, 陈伟, 等. 灵芝与大豆套种栽培技术[J]. 东南园艺, 2021, 9(1): 44-46. [74] 邢国辉, 王琦, 王希会, 等. 蘑菇培养基废料用作土壤改良剂: CN97121992. 3[P]. 1999-06-16. [75] 王家辰, 刘子嫣, 尹哲玉, 等. 农业生态系统减排增汇研究进展[J]. 中国生态环境保护进展, 2023, 1(3): 22-35. http://doi.org/10.48014/pceep.20230914001.
引用本文	尹哲玉, 王家辰, 亓香凝, 等. 农林废弃物资源化利用栽培灵芝技术进展[J]. 中国生态环境保护进展, 2024, 2(2): 11-21.
Citation	YIN Zheyu, WANG Jiachen, QI Xiangning, et al. The progress of Ganoderma lucidum cultivation by utilizing agricultural and forestry wastes[J]. Progress in Chinese Eco-Environmental Protection, 2024, 2(2): 11-21.