退化草原优势植物根际促生菌的筛选与功能分析

许祺龙; XU Qilong; 闫高俊; YAN Gaojun; 高博; GAO Bo; 王嘉山; WANG Jiashan; 白志辉; BAI Zhihui

doi:10.48014/pceep.20251023003

退化草原优势植物根际促生菌的筛选与功能分析

Screening and Functional Analysis of Plant Growth-Promoting Rhizobacteria from the Rhizosphere of Dominant Plant Species in Degraded Grasslands

中国生态环境保护进展 2025年第3卷第4期页码[37-45] 下载全文[2.8MB]

摘要

为挖掘退化草原植物根际促生菌 (plant growth-promoting rhizobacteria, PGPR) 资源, 本研究以内蒙古锡林浩特典型退化草原的羊草、麻花头、草麻黄、达乌里芯芭、细叶葱和大针茅6种优势植物根际土壤为材料, 通过稀释涂布法分离纯化根际细菌, 并评价其促生特性。实验共分离获得16株细菌, 其中12株具备两种及以上植物促生功能。功能分析显示, 7株具固氮能力, 10株具解钾能力, 6株具有产1-氨基环丙烷酸 (1-Amino cyclopropane carboxylic acid, ACC) 脱氨酶能力, 3株具有产铁载体能力, 2株具有产吲哚乙酸 (indole-3-acetic acid, IAA) 能力, 6株具有真菌拮抗能力。从中选取表现出良好的环境适应性和多重促生潜力的3株多功能菌株Y5、C1和X1, 经16S rRNA分子鉴定分别与Arthrobacter globiformis (KY649415. 1) 菌株、Brevibacterium frigoritolerans ( KC355256. 1) 菌株和Bacillus subtilis (MH373533. 1) 菌株同源相似性最高, 具备用于退化草原生态修复的微生物肥料开发潜力。本研究为退化草原植物和微生物的互作机制提供了新证据, 也为草原生态系统的可持续恢复提供了优良菌种资源。

Abstract

To explore the beneficial microbial resources in the rhizosphere of degraded grassland plants, this study used the rhizosphere soils of six dominant plants-Leymus chinensis, Chenopodium album , Pteridium aquilinum , Daurophyllum chinense, Microstegium vimineum , and Stipa grandis -from a typical degraded grassland in Xilinhot, Inner Mongolia as samples. Through dilution plating, the rhizosphere bacteria were isolated and purified, and their rhizosphere-promoting characteristics were systematically evaluated. A total of 16 bacterial strains were isolated, among which 12 strains had two or more rhizosphere-promoting functions. Functional analysis showed that 7 strains had nitrogen fixation ability, 10 strains had potassium solubilization ability, 6 strains had 1-aminocyclopropane carboxylic acid (ACC) deamination enzyme activity, 3 strains had iron carrier production ability, 2 strains had indole-3-acetic acid (IAA) production ability, and 6 strains had fungal antagonism ability. Three multi-functional bacterial strains Y5, C1 and X1 with good environmental adaptability and multiple rhizosphere-promoting potential were selected. Y5, C1 and X1 were identified by 16S rRNA molecular analysis and showed the highest homology similarity with Arthrobacter globiformis (KY649415. 1) , Brevibacterium frigoritolerans (KC355256. 1) , and Bacillus subtilis (MH373533. 1) , respectively, and had the potential for degraded grassland ecological restoration and microbial fertilizer development. This study provided new evidence for the interaction mechanism between degraded grassland plants and microorganisms and offered excellent bacterial strain resources for the sustainable recovery of grassland ecosystems.

DOI

10.48014/pceep.20251023003

文章类型

研究性论文

收稿日期

2025-10-23

接收日期

2025-11-29

出版日期

2025-12-28

关键词

植物根际促生菌(plant growth-promoting rhizobacteria, PGPR), 退化草原, 植物促生特性, 16s rRNA, 微生物肥料

Keywords

Plant growth-promoting rhizobacteria(PGPR), degraded grassland, plant growth-promoting characteristics, 16s rRNA, microbial fertiliser

作者

许祺龙^1,2, 闫高俊¹, 高博¹, 王嘉山¹, 白志辉^1,2,*

Author

XU Qilong^1,2, YAN Gaojun¹, GAO Bo¹, WANG Jiashan¹, BAI Zhihui^1,2,*

所在单位

1. 中国科学院生态环境研究中心, 北京 100085
2. 中国科学院大学中丹学院, 北京 100190

Company

1. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences (CAS) , Beijing 100085, China
2. Sino-Danish College, University of Chinese Academy of Sciences (UCAS) , Beijing 100190, China

浏览量

下载量

基金项目

国家重点研发计划课题(2023YFF1304101)和国家自然科学基金项目(42177111)资助

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引用本文

许祺龙, 闫高俊, 高博, 等. 退化草原优势植物根际促生菌的筛选与功能分析[J]. 中国生态环境保护进展, 2025, 3(4): 37-45.

Citation

XU Qilong, YAN Gaojun, GAO Bo, et al. Screening and functional analysis of plant growth-promoting rhizobacteria from the rhizosphere of dominant plant species in degraded grasslands[J]. Progress in Chinese Eco-Environmental Protection, 2025, 3(4): 37-45.