聚多巴胺涂层修饰海藻酸钙/聚丙烯酰胺防粘连水凝胶的制备及性能

薛贝贝; XUE Beibei; 王兆元; WANG Zhaoyuan; 董旭峰; DONG Xufeng

doi:10.48014/pcms.2022120202002

聚多巴胺涂层修饰海藻酸钙/聚丙烯酰胺防粘连水凝胶的制备及性能

Preparation and Properties of Polydopamine Coated Modified Calcium Alginate/Polyacrylamide Anti-adhesive Hydrogel

中国材料科学进展 2022年第1卷第3期页码[45-56] 下载全文[2.4MB] [HTML]

摘要

预防宫腔粘连 (IUA) 术后再粘连主要采用薄膜和水凝胶材料作为防粘连屏障, 但目前临床上使用的材料由于粘附性不足及降解太快等问题, 疗效仍不理想。海藻酸钙/聚丙烯酰胺 (CA/PAM) 双网络水凝胶力学性能及生物相容性优异, 适宜作为防粘连材料, 但粘附性不足, 易发生移位。本文采用聚多巴胺 (PDA) 涂层修饰CA/PAM水凝胶, 制备得到PDA@CA/PAM水凝胶, 以改善其粘附性。SEM图像表明PDA涂层成功涂覆, 并且水凝胶具有良好的三维多孔网络结构。力学性能测试显示水凝胶具有优异的拉伸及压缩性能, 使其可顺利进入目标位置并且承受一定的压力; 搭接-剪切实验证明, PDA涂层的修饰使CA/PAM水凝胶的组织粘附性能明显提升; 溶胀及降解性能表明该水凝胶可以吸收创面渗液并且在创面保持一定的时间。PDA涂层的引入也使得水凝胶的亲水性有所提升。细胞毒性测试及溶血实验显示, 水凝胶的细胞活性均在80%以上, 溶血率均在5%以下。并且PDA@CA/PAM水凝胶显示出比CA/PAM更高的细胞相容性和血液相容性。

Abstract

Film and hydrogel materials are mainly used as anti-adhesion barriers to prevent intrauterine adhesions (IUA) after surgery, but the efficacy of materials currently used in clinical practice is still not satisfactory due to insufficient adhesion and rapid degradation. Calcium alginate/polyacrylamide (CA/PAM) double-network hydrogel has excellent mechanical properties and biocompatibility, which is suitable for anti- adhesion materials, but the adhesion is insufficient and easy to shift. In this paper, polydopamine (PDA) coating was used to modify the CA/PAM hydrogel, and PDA@CA/PAM hydrogel was prepared to improve its adhesion properties. The SEM images show that the PDA coating is successfully applied, and that the hydrogel has a good three-dimensional porous network structure. The mechanical property test showed that the hydrogel had excellent tensile and compressibility, which allowed it to enter the target location smoothly and withstand certain pressure. The lap-shear test demonstrated that the modification of PDA coating significantly improved the tissue adhesion properties of CA/PAM hydrogels. The swelling and degradation properties indicated that the hydrogel could absorb the wound exudate and keep it on the wound for a certain period of time. The introduction of PDA coating also improved the hydrophilicity of the hydrogel. Cytotoxicity tests and hemolysis experiments showed that the cell activity of hydrogel was above 80%, and the hemolysis rate was below 5%. Moreover, PDA@CA/PAM hydrogel showed higher cell compatibility and blood compatibility than that of CA/PAM.

DOI	10.48014/pcms.2022120202002
文章类型	研究性论文
收稿日期	2022-12-02
接收日期	2022-12-12
出版日期	2022-12-28
关键词	防粘连, 水凝胶, 聚多巴胺, 海藻酸钠, 聚丙烯酰胺
Keywords	Anti-adhesion, hydrogel, polydopamine, sodium alginate, polyacrylamide
作者	薛贝贝¹, 王兆元², 董旭峰^1,*
Author	XUE Beibei¹, WANG Zhaoyuan², DONG Xufeng^1,*
所在单位	1. 大连理工大学材料科学与工程学院, 大连 116024 2. 首都医科大学附属北京友谊医院, 北京 100050
Company	1. School of Materials Science and Engineering, Dalian University of Technology, Dalian 116024, China 2. Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
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下载量	308
基金项目	本项研究得到了中央高校基本科研业务费(资助号DUT22YG201)的资助。
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引用本文	薛贝贝, 王兆元, 董旭峰. 聚多巴胺涂层修饰海藻酸钙/聚丙烯酰胺防粘连水凝胶的制备及性能[J]. 中国材料科学进展, 2022, 1(3): 45-56.
Citation	XUE Beibei, WANG Zhaoyuan, DONG Xufeng. Preparation and properties of polydopamine coated modified calcium alginate/polyacrylamide anti-adhesive hydrogel[J]. Progress in Chinese Materials Sciences, 2022, 1(3): 45-56.