聚多巴胺涂层修饰海藻酸钙/聚丙烯酰胺防粘连水凝胶的制备及性能

Preparation and Properties of Polydopamine Coated Modified Calcium Alginate/Polyacrylamide Anti-adhesive Hydrogel

预防宫腔粘连 (IUA) 术后再粘连主要采用薄膜和水凝胶材料作为防粘连屏障, 但目前临床上使用的材料由于粘附性不足及降解太快等问题, 疗效仍不理想。海藻酸钙/聚丙烯酰胺 (CA/PAM) 双网络水凝胶力学性能及生物相容性优异, 适宜作为防粘连材料, 但粘附性不足, 易发生移位。本文采用聚多巴胺 (PDA) 涂层修饰CA/PAM水凝胶, 制备得到PDA@CA/PAM水凝胶, 以改善其粘附性。SEM图像表明PDA涂层成功涂覆, 并且水凝胶具有良好的三维多孔网络结构。力学性能测试显示水凝胶具有优异的拉伸及压缩性能, 使其可顺利进入目标位置并且承受一定的压力; 搭接-剪切实验证明, PDA涂层的修饰使CA/PAM水凝胶的组织粘附性能明显提升; 溶胀及降解性能表明该水凝胶可以吸收创面渗液并且在创面保持一定的时间。PDA涂层的引入也使得水凝胶的亲水性有所提升。细胞毒性测试及溶血实验显示, 水凝胶的细胞活性均在80%以上, 溶血率均在5%以下。并且PDA@CA/PAM水凝胶显示出比CA/PAM更高的细胞相容性和血液相容性。

Film and hydrogel materials are mainly used as anti-adhesion barriers to prevent intrauterine adhesions (IUA) after surgery, but the efficacy of materials currently used in clinical practice is still not satisfactory due to insufficient adhesion and rapid degradation. Calcium alginate/polyacrylamide (CA/PAM) double-network hydrogel has excellent mechanical properties and biocompatibility, which is suitable for anti- adhesion materials, but the adhesion is insufficient and easy to shift. In this paper, polydopamine (PDA) coating was used to modify the CA/PAM hydrogel, and PDA@CA/PAM hydrogel was prepared to improve its adhesion properties. The SEM images show that the PDA coating is successfully applied, and that the hydrogel has a good three-dimensional porous network structure. The mechanical property test showed that the hydrogel had excellent tensile and compressibility, which allowed it to enter the target location smoothly and withstand certain pressure. The lap-shear test demonstrated that the modification of PDA coating significantly improved the tissue adhesion properties of CA/PAM hydrogels. The swelling and degradation properties indicated that the hydrogel could absorb the wound exudate and keep it on the wound for a certain period of time. The introduction of PDA coating also improved the hydrophilicity of the hydrogel. Cytotoxicity tests and hemolysis experiments showed that the cell activity of hydrogel was above 80%, and the hemolysis rate was below 5%. Moreover, PDA@CA/PAM hydrogel showed higher cell compatibility and blood compatibility than that of CA/PAM.