灵芝多糖对葡聚糖硫酸钠诱导小鼠溃疡性结肠炎的治疗作用研究

王朝阳; WANG Zhaoyang; 周黎黎; ZHOU Lili; 孙伟; SUN Wei; 钟小兰; ZHONG Xiaolan; 李旺林; LI Wanglin

doi:10.48014/atcm.20250925001

灵芝多糖对葡聚糖硫酸钠诱导小鼠溃疡性结肠炎的治疗作用研究

Study on the Therapeutic Effect of Ganoderma lucidum Polysaccharide on Dextran Sulfate Sodium Induced Ulcerative Colitis in Mice

中医药研究进展 2025年第1卷第3期页码[24-29] 下载全文[3MB]

摘要

本研究旨在探讨灵芝多糖(GLP)对溃疡性结肠炎(UC)的干预效果。通过建立葡聚糖硫酸钠(DSS)诱导的小鼠UC模型, 将实验动物随机分为正常对照组(Ctrl)、DSS模型组(DSS)以及GLP处理组, 并系统评估各组小鼠的体重变化、疾病活动指数(DAI)、结肠长度、组织病理学特征及炎症因子表达水平, 以综合评价GLP的治疗潜力。结果显示: 与DSS组相比, GLP干预可显著缓解小鼠体重下降、结肠缩短, 降低DAI评分; HE染色表明GLP改善结肠黏膜损伤并减少炎症细胞浸润; qPCR显示GLP下调结肠黏膜中促炎因子Il-6和Tnf-α的mRNA表达。综上, GLP可通过抑制肠道炎症, 改善DSS诱导的小鼠UC症状及病理损伤, 为其后续研究与应用提供实验依据。

Abstract

This study aims to investigate the interventional effects of Ganoderma lucidum polysaccharide (GLP) on ulcerative colitis (UC) . A mouse model of UC was established by induction with dextran sulfate sodium (DSS) . The experimental animals were randomly divided into a normal control group (Ctrl) , a DSS model group (DSS) , and a GLP treatment group. A systematic evaluation was conducted to assess changes in body weight, disease activity index (DAI) , colon length, histopathological features, and inflammatory cytokine expression levels in mice from each group to comprehensively evaluate the therapeutic potential of GLP. The results showed that, compared to the DSS group, GLP intervention significantly alleviated body weight loss, colon shortening, and decreased the DAI score. H&E staining indicated that GLP improved colonic mucosal damage and reduced inflammatory cell infiltration. qPCR results demonstrated that GLP down regulated the mRNA expression of the pro-inflammatory factors Il-6 and Tnf-α in the colonic mucosa. In conclusion, GLP can alleviate DSS-induced UC symptoms and pathological damage in mice by suppressing intestinal inflammation, providing an experimental basis for its further research and application.

DOI

10.48014/atcm.20250925001

文章类型

研究性论文

收稿日期

2025-09-25

接收日期

2025-10-27

出版日期

2025-12-28

关键词

溃疡性结肠炎, DSS, 灵芝多糖, 炎症因子

Keywords

Ulcerative colitis, DSS, Ganoderma Lucidum Polysaccharide, inflammatory factors

作者

王朝阳^1,2,*, 周黎黎², 孙伟², 钟小兰², 李旺林^2,3

Author

WANG Zhaoyang^1,2,*, ZHOU Lili², SUN Wei², ZHONG Xiaolan², LI Wanglin^2,3

所在单位

1. 广州医科大学公共卫生学院, 广州 511436
2. 广州市花都区人民医院花都医学研究所, 广州 510800
3. 广州市花都区人民医院胃肠外科, 广州 510800

Company

1. School of Public Health, Guangzhou Medical University, Guangzhou 511436, China
2. Huadu Medical Research Institute, Huadu District People' s Hospital of Guangzhou, Guangzhou 510800, China
3. Department of Gastrointestinal Surgery, Huadu District People' s Hospital of Guangzhou, Guangzhou 510800, China

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基金项目

本项研究得到了花都区基础与应用基础研究区院联合资助项目(项目编号:25HDQYLH03)的资助

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引用本文

王朝阳,周黎黎,孙伟,等.灵芝多糖对葡聚糖硫酸钠诱导小鼠溃疡性结肠炎的治疗作用研究[J].中医药研究进展,2025,1(3):24-29.

Citation

WANG Zhaoyang, ZHOU Lili, SUN Wei, et al. Study on the therapeutic effect of Ganoderma lucidum Polysaccharide on dextran sulfate sodium induced ulcerative colitis in mice[J]. Advances in Traditional Chinese Medicine, 2025, 1(3): 24-29.