植物组织培养技术是中等职业学校园林专业学生须掌握的基本技能。分析职业学校在植物细胞工程模块学习方面存在“重理论, 轻实践”的问题以及组培模块实验改进的需求后, 本研究依据职业高中学校的培养方案, 对植物细胞工程模块进行改进。包括引入实验课程和项目实践。在实施过程中, 将理论知识与实践操作相结合, 以“马铃薯组织培养”实验为例, 围绕“培养基配置、无菌接种、结薯率和试管苗再繁结果观察”三阶段来提升学生的专业素养。实践中, 学生利用组织培养技术成功地获取了马铃薯的愈伤组织, 并进行了植株再生和快速繁殖等实验。通过实践操作, 学生深入了解植物细胞工程的基本原理和实际应用, 同时培养他们的实验技能和科学素养。结果表明, 改进的教学方法和实践操作对提高职业高中学生的学习非常有效。学生在实践中不仅增加对植物细胞工程技术的理解和掌握, 还提高了实验技能和科学思维能力; 同时, 在团队合作和创新意识方面得到了锻炼和提升。本研究为职业高中植物细胞工程模块的教学改进提供了有益的经验和启示。

Plant tissue culture technology is a basic skill that vocational high school landscape architecture students must master. After analyzing the problem of “emphasizing theory over practice” in the learning of plant cell engineering modules in vocational schools and the need for experimental improvement of tissue culture modules, this study improved the plant cell engineering module according to the culture scheme of vocational high schools, including the introduction of lab courses and project practice. In the implementation process, the theoretical knowledge and practical operation are combined, taking the “potato tissue culture” experiment as an example, focusing on the three stages of “medium configuration, sterile inoculation, potato rate and observation of test tube seedling remultiplication results” to improve students􀆳 professional quality. In practice, students successfully obtain calluses from potatoes using tissue culture technology, and conduct experiments such as plant regeneration and rapid propagation. Through hands-on practice, students gain an in-depth understanding of the basic principles and practical applications of plant cell engineering while developing their experimental skills and scientific literacy. The results show that the improved teaching methods and practical operations are very effective in improving the learning of vocational high school students. In practice, students not only increase their understanding and mastery of plant cell engineering technology, but also improve their experimental skills and scientific thinking ability. At the same time, they have been exercised and improved in terms of teamwork and innovative awareness. This study provides useful experience and enlightenment for the teaching improvement of plant cell engineering modules in vocational high schools.